RIDAO

RIDAO (Rapid Intrinsic Disorder Analysis Online): Integrated high-throughput protein disorder prediction

RIDAO predicts intrinsically disordered proteins (IDPs) and disordered regions at per-residue resolution by integrating six established disorder prediction algorithms. It reproduces individual predictor outputs while enabling genome-scale intrinsic disorder analysis.

Key Features:

• Multi-Predictor Integration: Combines six established intrinsic disorder predictors into a unified computational framework with high fidelity to original methods.
• High-Throughput Performance: Generates per-residue disorder predictions two to six orders of magnitude faster than AUCpreD, IUPred3, metapredict V2, flDPnn, and SPOT-Disorder2.
• Large-Scale Scalability: Processes datasets exceeding one million protein sequences (>420 million residues) from 100 organisms in under one hour.

Scientific Applications:

• Genome-Scale Structural Bioinformatics: Enables comparative genomics and proteomics analyses of intrinsic disorder across diverse organisms and large proteome datasets.

Methodology:

RIDAO executes and consolidates predictions from six established intrinsic disorder algorithms within a unified pipeline, producing per-residue disorder scores suitable for large-scale structural and comparative analyses.