RJunBase

RJunBase catalogs RNA splice junctions from RNA-seq data to enable comparative analysis of linear, back-splice, and fusion junctions across normal and cancerous human tissues.

Key Features:

• Data Integration: Integrates RNA sequencing (RNA-seq) data from 18,084 non-cancerous and 11,540 cancerous human tissue samples for junction discovery and comparison.
• Splice Junction Types: Records three junction types with definitions: linear junctions resulting from intron removal in multi-exon genes; back-splice junctions that connect the 3' end of an exon to the 5' end of a downstream exon; and fusion junctions where exons from different genes are joined.
• Database Content: The initial release catalogs 682,017 linear junctions, 225,949 back-splice junctions, and 34,733 fusion junctions.
• Visualization and Analysis: Provides detailed visualization of gene-level splicing patterns and junction-level expression profiles across tissue types.

Scientific Applications:

• Unannotated Junction Discovery: Identifies unannotated splice junctions and tumor-specific alterations to support molecular characterization of disease.
• Biomarker Discovery: Supports discovery of splicing-associated biomarkers for research in genomics and oncology.
• Neoepitope Assessment: Aids identification and assessment of neoepitopes relevant to cancer treatment.

Methodology:

Integration of RNA-seq data from 18,084 non-cancerous and 11,540 cancerous human tissue samples to identify and catalog linear, back-splice, and fusion splice junctions.