rnaQUAST

rnaQUAST evaluates RNA-Seq transcriptome assemblies by computing completeness and correctness metrics through reference-based comparison with genome and gene databases.

Key Features:

• Assembly Quality Metrics: Calculates quantitative measures of transcriptome assembly completeness and correctness, including coverage of known genes and accuracy of gene structure representation.
• Reference-Based Benchmarking: Aligns assembled transcripts to a reference genome and gene database to assess assembly fidelity and identify structural errors or omissions.

Scientific Applications:

• Transcriptome Assembly Evaluation: Enables objective comparison of RNA-Seq assembly methods to support gene expression analysis, functional annotation, and comparative genomics.

Methodology:

rnaQUAST aligns assembled transcripts against a reference genome and gene database and computes metrics quantifying gene coverage, structural accuracy, and overall assembly quality to assess transcriptome reconstruction performance.