RNAither

RNAither analyzes RNA interference (RNAi) screening data to identify significant siRNAs and associated biological processes.

Key Features:

• Quality Assessment and Normalization: Performs signal intensity quality control and applies multiple normalization methods to accommodate diverse experimental designs.
• Statistical and Functional Analysis: Applies statistical tests to detect significant siRNAs and conducts biological process enrichment analysis for affected genes.

Scientific Applications:

• RNAi Functional Genomics: Supports identification of gene functions, pathways, and interaction networks from high-throughput RNAi screens.

Methodology:

RNAither processes RNAi screening datasets through quality control, normalization, statistical testing for siRNA significance, and downstream biological process enrichment analysis within the R/Bioconductor framework.