RNAPhaSep

RNAPhaSep provides a curated database of experimentally validated instances of RNA-involved liquid-liquid phase separation (LLPS) to support study of RNA roles in membraneless organelle formation and disease-related phase behavior.

Key Features:

• Manually curated dataset: 1,113 entries representing experimentally validated instances of RNA self-assembly or RNA–protein co-involvement in phase separation, curated from published literature and public databases.
• Detailed molecular context: Entries include RNA information, interacting proteins (including RNA-binding proteins), and specific experimental conditions under which LLPS occurs.
• Experimental validation: Each entry is supported by experimental evidence documenting RNA-induced or RNA-involving phase separation events.
• Integrated annotations: Annotations from multiple databases are incorporated to provide enriched biological context for each entry.

Scientific Applications:

• Study of RNA-mediated LLPS: Enable investigation of how RNAs seed or nucleate membraneless organelles via phase separation.
• Analysis of sequence–function relationships: Facilitate analysis of relationships between RNA properties (e.g., sequence, motifs) and phase behavior.
• Investigation of RNA–protein co-phase separation: Support examination of interactions between RNAs and proteins in co-assembly and granule formation.
• Disease and cellular function research: Provide data to explore implications of RNA-mediated LLPS in cellular processes and human diseases and to generate hypotheses for therapeutic strategies.

Methodology:

Dataset assembled by manual curation from published literature and public databases, integrating annotations from multiple databases to compile 1,113 experimentally validated RNA-involved LLPS entries.