RNASequest

RNASequest automates reproducible RNAseq differential gene expression analysis, covariates testing, and centralized management of RNAseq datasets for downstream visualization with Quickomics.


Key Features:

  • Reproducible Expression Analysis Module: Automates differential gene expression analysis via a configurable expression analysis (EA) module and includes built-in covariates testing for statistical model design.
  • Centralized Data Management System: Provides integrated handling and organization of RNAseq datasets across multiple projects to support consistent data oversight.
  • Quickomics Integration: Integrates with Quickomics to enable downstream visualization and exploration of analysis results.

Scientific Applications:

  • Differential Gene Expression Studies: Performs automated differential expression analyses with covariate testing to support rigorous statistical comparisons.
  • Multi-project RNAseq Analyses: Organizes and manages RNAseq datasets across projects to facilitate comparative and longitudinal investigations.
  • Result Visualization and Exploration: Supports downstream visualization and exploratory analysis of results via Quickomics.

Methodology:

Automation through a configurable expression analysis module that performs differential gene expression analysis with built-in covariates testing, combined with a centralized data management system, emphasizing automation and customization.

Topics

Details

License:
MIT
Cost:
Free of charge
Tool Type:
command-line tool
Operating Systems:
Mac, Linux, Windows
Programming Languages:
R, Shell
Added:
3/18/2023
Last Updated:
11/24/2024

Operations

Publications

Zhu J, Sun YH, Ouyang Z, Li K, Negi S, Piya S, Hu W, Zavodszky MI, Yalamanchili H, Chen Y, Zhang X, Casey F, Zhang B. RNASequest: An End-to-End Reproducible RNAseq Data Analysis and Publishing Framework. Journal of Molecular Biology. 2023;435(14):168017. doi:10.1016/j.jmb.2023.168017. PMID:36806691.

Documentation