RNASequest
RNASequest automates reproducible RNAseq differential gene expression analysis, covariates testing, and centralized management of RNAseq datasets for downstream visualization with Quickomics.
Key Features:
- Reproducible Expression Analysis Module: Automates differential gene expression analysis via a configurable expression analysis (EA) module and includes built-in covariates testing for statistical model design.
- Centralized Data Management System: Provides integrated handling and organization of RNAseq datasets across multiple projects to support consistent data oversight.
- Quickomics Integration: Integrates with Quickomics to enable downstream visualization and exploration of analysis results.
Scientific Applications:
- Differential Gene Expression Studies: Performs automated differential expression analyses with covariate testing to support rigorous statistical comparisons.
- Multi-project RNAseq Analyses: Organizes and manages RNAseq datasets across projects to facilitate comparative and longitudinal investigations.
- Result Visualization and Exploration: Supports downstream visualization and exploratory analysis of results via Quickomics.
Methodology:
Automation through a configurable expression analysis module that performs differential gene expression analysis with built-in covariates testing, combined with a centralized data management system, emphasizing automation and customization.
Topics
Details
- License:
- MIT
- Cost:
- Free of charge
- Tool Type:
- command-line tool
- Operating Systems:
- Mac, Linux, Windows
- Programming Languages:
- R, Shell
- Added:
- 3/18/2023
- Last Updated:
- 11/24/2024
Operations
Publications
Zhu J, Sun YH, Ouyang Z, Li K, Negi S, Piya S, Hu W, Zavodszky MI, Yalamanchili H, Chen Y, Zhang X, Casey F, Zhang B. RNASequest: An End-to-End Reproducible RNAseq Data Analysis and Publishing Framework. Journal of Molecular Biology. 2023;435(14):168017. doi:10.1016/j.jmb.2023.168017. PMID:36806691.