RPGD

RPGD provides centralized storage and integrated analysis of genomic and transcriptomic data for Rhododendron species to support genome assembly, annotation, expression profiling, and comparative genomics.


Key Features:

  • Genome assemblies: Complete genome sequence assemblies for R. delavayi, R. williamsianum, and R. simsii.
  • Gene expression profiles: Integrated gene expression profiles derived from public RNA-Seq datasets.
  • Functional annotation: Extensive functional annotations of genes.
  • Gene families and transcription factors: Identification and cataloging of gene families and transcription factors.
  • Gene homology and Orthologous Groups: Gene homology information and Orthologous Groups for evolutionary analysis.
  • Simple sequence repeats (SSRs): Identification of simple sequence repeats (SSRs).
  • Chloroplast genomes: Inclusion of chloroplast genome sequences and related data.
  • BLAST: BLAST for sequence alignment and similarity searches.
  • JBrowse: JBrowse for genome visualization.
  • Genome Synteny Browser: Genome Synteny Browser to explore chromosomal rearrangements.
  • Flanking Sequence Finder: Flanking Sequence Finder to locate adjacent genomic regions.
  • Expression Heatmap: Expression Heatmap for visualization of gene expression data.
  • Batch Download: Batch Download for bulk retrieval of datasets.
  • Data integration: Integration of genomics and transcriptomics datasets from public sources.

Scientific Applications:

  • Genome-based gene discovery: Discovery and annotation of genes in Rhododendron genomes.
  • Transcriptome analysis: Transcriptome profiling and visualization using RNA-Seq-derived expression data.
  • Comparative genomics: Comparative genomics and synteny analysis across Rhododendron species.
  • Evolutionary and orthology studies: Evolutionary analysis using orthologous groups and gene homology information.

Methodology:

Integration of complete genome sequence assemblies and public RNA-Seq datasets for gene expression profiles, functional annotation, identification of gene families and transcription factors, detection of gene homology, simple sequence repeats and chloroplast genomes; supported computational tools and components include BLAST, JBrowse, Orthologous Groups, Genome Synteny Browser, Flanking Sequence Finder, Expression Heatmap, and Batch Download.

Topics

Details

Tool Type:
web application
Operating Systems:
Mac, Linux, Windows
Added:
11/29/2021
Last Updated:
11/29/2021

Operations

Publications

Liu N, Zhang L, Zhou Y, Tu M, Wu Z, Gui D, Ma Y, Wang J, Zhang C. The Rhododendron Plant Genome Database (RPGD): a comprehensive online omics database for Rhododendron. BMC Genomics. 2021;22(1). doi:10.1186/s12864-021-07704-0. PMID:34022814. PMCID:PMC8141123.

PMID: 34022814
PMCID: PMC8141123
Funding: - National Natural Science Foundation of China: 31760231 - Construction of International Flower Technology Innovation Center and Industrialization of achievements: 2019ZG006 - Program of Science and Technology Talents Training in Yunnan province: 2016HA005 - Youth Program of National Natural Science Foundation of China: 32000180