RPiso

RPiso analyzes ribosome profiling (Ribo-seq) data at the mRNA isoform level to estimate isoform-specific translation and abundance.

Key Features:

• Isoform-Level Abundance Estimation: Quantifies ribosome-protected fragment distributions to provide accurate estimates of mRNA isoform translation abundance.
• Multi-Species Ribo-seq Support: Processes ribosome profiling datasets across multiple species to enable comparative analysis of translational regulation.

Scientific Applications:

• Isoform-Specific Translational Regulation Analysis: Investigates differential translation of mRNA isoforms to study gene expression control and disease-associated translational dysregulation.

Methodology:

RPiso implements a computational pipeline that maps ribosome-protected mRNA fragments to transcript isoforms, quantifies isoform-specific ribosome occupancy, and estimates translation abundance using large-scale ribosome profiling datasets.