RS-FISH

RS-FISH detects and localizes diffraction-limited single-molecule fluorescent in-situ hybridization (FISH) spots in two-dimensional and three-dimensional microscopy images to enable precise spatial analysis of molecular processes.

Key Features:

• Accuracy and Robustness: Detects single-molecule FISH (smFISH) spots with high precision across two-dimensional and three-dimensional datasets, providing reliable identification in complex imaging conditions.
• Parameter Tuning: Supports adjustment of detection parameters to optimize spot identification for specific experimental conditions and sample types.
• Scalability: Processes large-scale image datasets and supports distributed processing for analysis of extensive image volumes.

Scientific Applications:

• Single-Molecule FISH (smFISH): Enables study of gene expression at single-molecule resolution within cells.
• Spatial Transcriptomics: Facilitates mapping of RNA transcripts across tissue sections to reveal spatial gene expression patterns.
• Spatial Genomics: Aids localization and analysis of genomic elements within their native cellular context.

Methodology:

Uses the Radial Symmetry algorithm, analyzing symmetry around potential spot centers to distinguish true molecular signals from background noise.