RSEQtools

RSEQtools analyzes RNA-Seq data using the Mapped Read Format (MRF) to enable anonymized functional genomics analysis.

Key Features:

• Mapped Read Format (MRF) Anonymization: Uses MRF to summarize mapped reads while preventing disclosure of individual-specific sequence information.
• Modular RNA-Seq Analysis: Provides modules for gene expression quantification (Reads Per Kilobase Million, RPKM), signal track generation, and segmentation of actively transcribed regions.

Scientific Applications:

• Privacy-Preserving Transcriptome Analysis: Supports RNA-Seq expression profiling and functional genomics studies in data-sharing environments requiring sequence anonymization.

Methodology:

RSEQtools processes mapped RNA-Seq reads in MRF format, decoupling alignment from downstream analysis, and applies quantitative expression calculation and signal segmentation algorithms within a modular C-based framework.