Rust Expression Browser

Rust Expression Browser provides simultaneous analysis of host and pathogen gene expression from RNA-Seq data to study interactions between wheat and Puccinia striiformis f. sp. tritici (Pst).

Key Features:

• Dual RNA-Seq Approach: Implements a dual RNA-Seq methodology referred to as field pathogenomics to capture gene expression from both pathogen and host simultaneously.
• Extensive Dataset Integration: Integrates 538 new RNA-Seq datasets generated via field pathogenomics, 66 Pst infection time-course datasets, and 420 publicly available datasets, totaling 1,024 processed RNA-Seq datasets from field and laboratory samples.
• Largest Database for Wheat Rust Pathogens: Hosts the largest collection of processed RNA-Seq datasets available for any of the three Puccinia wheat rust pathogens.

Scientific Applications:

• Pathogen Surveillance and Virulence Studies: Enables analysis of temporal expression of putative Pst virulence genes to support surveillance and studies of pathogen adaptation.
• Host Response Analysis: Enables detailed examination of wheat transcriptional responses to Pst infection to investigate host defense mechanisms.

Methodology:

Dual RNA-Seq (field pathogenomics) processing and integration of 1,024 RNA-Seq datasets from field and laboratory samples.