SAGE Home Page

SAGE Home Page provides quantitative transcriptome profiling using Serial Analysis of Gene Expression (SAGE) short diagnostic sequence tags to catalog and compare gene expression across biological samples.

Key Features:

• Quantitative Analysis: Precise quantification of gene expression by isolating short sequence tags from mRNA and generating libraries through concatenation and cloning for tag counting.
• Simultaneous Expression Profiling: Concurrent analysis of multiple genes by analyzing large numbers of diagnostic sequence tags to assess relative transcript abundance.
• Broad Applicability: Use across normal physiological states, developmental processes, and disease conditions for comparative gene expression studies.
• Discovery of Novel Transcripts: Identification of previously unrecognized transcripts based on unique short diagnostic tag sequences.

Scientific Applications:

• Organ- and disease-specific expression analysis: Characterization of gene expression patterns associated with specific organ functions or disease states.
• Pancreatic tissue profiling: Application in pancreatic tissue to reveal expression profiles and identify novel transcripts relevant to pancreatic biology.

Methodology:

Sequencing and analysis of concatenated short diagnostic sequence tags are used to quantify transcript abundance and identify novel transcripts.