The SBT (Sequencing-based typing) interface

The SBT (Sequencing-based typing) interface evaluates the allelic composition of SBT-derived cDNA or genomic sequences to identify and compare sample HLA and KIR alleles against dbMHC reference alleles.

Key Features:

• Reference database: Uses dbMHC (NCBI) containing curated allele sequences for multiple human leukocyte antigen (HLA) and killer cell immunoglobulin-like receptor (KIR) loci.
• Allele identification: Evaluates sequencing-based typing (SBT) data to generate candidate allele matches for samples.
• Sample type support: Accepts heterozygous, haploid, or combined sample genotypes for analysis.
• Match reporting: Produces a detailed results table listing potential allele matches for each sample.
• Base-change annotation: Reports specific nucleotide differences associated with each potential allele hit.
• Sequence alignment viewer: Provides examination of sample sequence alignments against reference alleles to verify matches.

Scientific Applications:

• Immunogenetics: Determination of HLA and KIR allelic composition for studies of immune system genetics and variation.
• Transplant compatibility: Allele-level typing to support assessment of donor–recipient HLA matching and KIR considerations.
• Disease association and personalized medicine: Identification of alleles relevant to disease susceptibility, vaccine response, and individualized clinical decision-making.

Methodology:

Aligns sample cDNA or genomic sequences against dbMHC reference alleles and reports potential allele matches with annotated nucleotide differences in a results table.