scMNase-seq

scMNase-seq profiles genome-wide nucleosome positioning and chromatin accessibility at single-cell resolution using micrococcal nuclease (MNase) digestion.

Key Features:

• Simultaneous Profiling: Detects nucleosome positioning and chromatin accessibility concurrently from single cells via MNase digestion.
• Fragment-size Resolution: Recovers subnucleosome-sized (≤80 bp) fragments for accessibility and mononucleosome-sized (140–180 bp) fragments for precise nucleosome positioning.
• Single-Cell Isolation: Initiates from individual cells isolated by fluorescence-activated cell sorting (FACS).
• Library Preparation: DNA fragments are purified, end-repaired, ligated to Y-shaped adaptors, and PCR-amplified using indexing primers.
• Sequencing and Yield: Libraries are sequenced on Illumina HiSeq platforms with typical output of ~0.5–1 million unique mapped reads per single cell.
• Enzymatic Specificity: Uses micrococcal nuclease (MNase) which preferentially cleaves DNA not protected by nucleosomes.

Scientific Applications:

• Chromatin Dynamics and Epigenetic Regulation: Enables study of nucleosome organization and epigenetic state variation at single-cell resolution.
• Cell-to-Cell Heterogeneity: Facilitates analysis of variability in chromatin states across individual cells within a population.
• Development and Disease Research: Applicable to investigations of developmental biology, cancer heterogeneity, and other complex biological systems.

Methodology:

No computational processing steps are specified in the provided description.