ScanProsite

ScanProsite detects PROSITE signature matches in protein sequences and annotates motifs, protein families, and functional or structural intra-domain residues using PROSITE patterns and ProRules.

Key Features:

• Implementation of PROSITE Syntax: Adheres strictly to the PROSITE-defined syntax and matching rules for pattern detection.
• ProRules integration: Incorporates ProRules context-dependent annotation templates to identify functional and structural intra-domain residues within PROSITE profiles.
• Search scope and pre-computed matches: Scans user-provided sequences and UniProt Knowledgebase (UniProtKB) entries against custom patterns or established PROSITE signatures and retrieves pre-computed match results for UniProtKB sequences.
• ExPASy integration: Provided as part of the ExPASy suite.

Scientific Applications:

• Protein family classification: Detects PROSITE signatures to classify proteins into families and identify conserved motifs.
• Motif discovery: Identifies PROSITE patterns and custom sequence motifs within protein sequences.
• Functional and structural annotation: Predicts functional sites and structural intra-domain residues via PROSITE matches combined with ProRules annotations, supporting structure–function analyses in molecular biology, biochemistry, and genomics.

Methodology:

Pattern matching using PROSITE-defined syntax and rules; incorporation of ProRules context-dependent annotation templates to assign functional and structural intra-domain residues within PROSITE profiles; scanning of user sequences and UniProtKB entries against custom patterns or established PROSITE signatures; retrieval of pre-computed match results for UniProtKB sequences.