SCcaller

SCcaller identifies single-nucleotide variants (SNVs) and short insertions and deletions (INDELs) from single-cell sequencing data to enable accurate somatic mutation analysis while mitigating artifacts introduced by cell lysis and whole-genome amplification.

Key Features:

• Artifact Mitigation: SCcaller reduces artifacts associated with cell lysis and whole-genome amplification to improve accuracy of SNV and INDEL detection.
• Integration with SCMDA: SCcaller leverages single-cell multiple displacement amplification (SCMDA) to provide a more reliable basis for variant calling and somatic-mutation analysis.
• General-Purpose Variant Calling: SCcaller performs general-purpose variant calling for single-cell genomic data applicable across diverse research contexts.

Scientific Applications:

• Cancer Genomics: Enables analysis of somatic mutations within individual cells to study tumor heterogeneity and evolution.
• Developmental Biology: Supports detailed genetic analyses of single cells during organismal development.

Methodology:

Validation by comparative analysis between SCMDA-amplified single cells and unamplified clones derived from identical cell populations to assess accuracy of detected variants.