Scipion

Scipion integrates multiple three-dimensional electron microscopy (3DEM) software packages into workflow-based pipelines for standardized, traceable, and reproducible 3DEM image processing.

Key Features:

• Integration of Multiple Software Packages: Combines tools from different 3DEM programs to enable interoperability across software packages.
• Workflow-Based Approach: Implements reusable, standardized processing workflows composed of sequential image-processing steps.
• Reusability and Standardization: Provides protocols that can be reused across projects to promote consistent processing and comparable results.
• Traceability and Reproducibility: Documents each processing step within workflows to enable verification and replication of analyses.

Scientific Applications:

• Structural biology: Processes 3DEM data for determination of macromolecular structures such as proteins.
• Protein and macromolecular complex analysis: Facilitates image processing for proteins and other macromolecular assemblies imaged by 3DEM.
• Cellular component imaging: Enables reconstruction and analysis of cellular components imaged with 3DEM.
• Materials science: Applies standardized 3DEM image-processing workflows to problems in materials science.

Methodology:

Creates customizable workflows that incorporate tools from multiple 3DEM software packages and documents each processing step to maintain interoperability, traceability, and reproducibility.