SCITO

SCITO leverages DNA-barcoded antibodies and splint oligonucleotides to enable ultra-high-throughput, multiplexed profiling of surface proteins and simultaneous transcriptome measurement in droplet-based single-cell sequencing (dsc-seq) workflows.

Key Features:

• Splint oligonucleotides and combinatorial indexing: Uses splint oligos to perform combinatorial indexing that increases cell throughput per reaction.
• High-throughput capacity: Profiles protein abundances from over 100,000 cells per reaction on commercial microfluidics platforms.
• Sample barcode encoding: Encodes sample barcodes into splint oligos to enable multiplexed sequencing of pooled samples.
• Compatibility with DNA-barcoded antibodies: Provides two modified splint oligo designs to enhance compatibility with commercial DNA-barcoded antibodies.
• Multimodal profiling: Enables simultaneous profiling of the transcriptome and surface proteins from individual cells.
• Quantitative cell composition and protein expression: Produces reproducible estimates of cellular composition and surface protein expression comparable to mass cytometry.
• Cost-efficiency for large-scale studies: Addresses high per-cell cost of standard dsc-seq by increasing multiplexing and throughput for pooled screens and clinical-scale experiments.

Scientific Applications:

• Large-scale pooled screens: Enables high-throughput protein readouts across many samples in pooled experimental designs.
• Clinical and translational studies: Reduces per-cell cost and increases throughput to support clinical-scale single-cell protein profiling.
• Multimodal single-cell analysis: Integrates surface protein and transcriptome measurements from the same cells for comprehensive cellular phenotyping.
• Comparative benchmarking: Provides protein quantification comparable to mass cytometry for validation and benchmarking of single-cell assays.
• Ultra-high-throughput protein analysis: Supports comprehensive single-cell protein profiling at population scales enabled by droplet-based microfluidics.

Methodology:

SCITO uses splint oligonucleotides for combinatorial indexing, encodes sample barcodes into splint oligos attached to DNA-barcoded antibodies (including two modified designs), and processes samples on commercial droplet-based single-cell sequencing microfluidics for multiplexed protein and transcriptome profiling.