scPlant

scPlant provides a framework for analysis of plant single-cell transcriptomic data, enabling processing, cell-type annotation and deconvolution, trajectory inference, cross-species integration, and gene regulatory network construction to study plant growth, development, and responses to environmental stimuli.


Key Features:

  • End-to-End Pipeline: Implements a comprehensive pipeline for exploration of plant single-cell atlases from minimal input data.
  • Basic Data Processing: Handles and preprocesses raw single-cell transcriptomic data.
  • Cell-Type Annotation and Deconvolution: Identifies and classifies cell types within plant tissue samples.
  • Trajectory Inference: Reconstructs developmental trajectories to characterize cellular differentiation processes.
  • Cross-Species Data Integration: Integrates data across different plant species to facilitate comparative studies.
  • Gene Regulatory Network Construction: Builds cell-type-specific gene regulatory networks to elucidate genetic control mechanisms.

Scientific Applications:

  • Cellular Heterogeneity Analysis: Investigates cellular heterogeneity within plant tissues at single-cell resolution.
  • Developmental Process Analysis: Studies developmental processes and cellular differentiation at the single-cell level.
  • Stress Response Analysis: Examines plant responses to biotic and abiotic stresses through cell-type-specific analyses.
  • Comparative and Evolutionary Studies: Enables cross-species comparisons to identify conserved genetic pathways.

Methodology:

Performs basic data processing and preprocessing of raw single-cell transcriptomic data, cell-type annotation and deconvolution, trajectory inference, cross-species data integration, and gene regulatory network construction.

Topics

Details

License:
GPL-3.0
Cost:
Free of charge
Tool Type:
desktop application, workflow
Operating Systems:
Mac, Linux, Windows
Programming Languages:
R
Added:
1/2/2024
Last Updated:
11/24/2024

Operations

Publications

Cao S, He Z, Chen R, Luo Y, Fu L, Zhou X, He C, Yan W, Zhang C, Chen D. scPlant: A versatile framework for single-cell transcriptomic data analysis in plants. Plant Communications. 2023;4(5):100631. doi:10.1016/j.xplc.2023.100631. PMID:37254480. PMCID:PMC10504592.

PMID: 37254480
Funding: - National Natural Science Foundation of China: 32070656

Links