ScreenGarden

ScreenGarden performs statistical analysis and plate-to-plate comparison of colony growth measurements from plate-based high-throughput genomic screens.

Key Features:

• Plate-based colony analysis: Quantitative analysis of colony growth measurements on solid media from high-throughput screens.
• Control comparisons: Statistical comparison between experimental and control plates to identify differential growth phenotypes.
• Technical artifact handling: Detection and mitigation of effects from pinning errors and plate-specific growth variability to reduce false positives.
• Imaging-derived data processing: Processing of colony size measurements generated by imaging software for downstream analysis.
• High-throughput performance: Enables rapid analysis workflows suitable for large plate-based datasets.

Scientific Applications:

• Yeast-two hybrid assays: Analysis of colony growth readouts to assess protein–protein interaction outcomes.
• Synthetic Genetic Arrays (SGA): Comparative analysis of colony sizes to identify genetic interaction phenotypes.
• Synthetic Physical Interaction (SPI) screens: Evaluation of colony growth patterns to detect synthetic physical interactions.
• High-throughput genomic screens: General application to plate-based genomic experiments requiring quantitative colony growth comparison.

Methodology:

Performs statistical analyses and plate-to-plate comparisons of imaging-derived colony size measurements while accounting for pinning errors and plate-specific growth effects.