screenwerk

screenwerk facilitates the design and analysis of high-throughput drug combination screens as a modular R package to identify synergistic and antagonistic interactions and to profile drug sensitivity in cell lines and patient-derived samples.

Key Features:

• Modularity and Customization: Modular R package architecture allows customization of analysis pipelines for specific experimental designs.
• End-to-End Pipeline: Provides functions for experimental design, setting up drug sensitivity screens, reading raw measurements, consolidating datasets, and analyzing results.
• Quality Control and Synergy Analysis: Includes flexible quality-control procedures and supports identification of synergistic and antagonistic interactions, including integration with bayesynergy.
• Scalability: Designed to handle large-scale datasets across numerous cell lines or patient-derived samples.
• Integration with Other R Packages: Integrates with R packages such as bayesynergy to extend analytical capabilities.
• Data Reporting and Visualization: Provides reporting and visualization modules for summarizing results from screens and combination analyses.

Scientific Applications:

• Drug Sensitivity Screening: Setup and analysis of drug sensitivity and dose–response screens to determine efficacy of compounds and combinations in cell lines and patient-derived samples.
• Synergy Detection: Detection and characterization of synergistic and antagonistic drug interactions, including analysis with bayesynergy.
• Precision Medicine and Combination Therapy Development: Support for identifying effective combination therapies in cancer and infectious disease contexts and for precision medicine applications.

Methodology:

Modular pipeline construction supporting experimental design, reading raw measurements, dataset consolidation, quality control, analysis of drug combination responses, and integration with bayesynergy.