scTail

scTail identifies polyadenylation sites using first-strand reads and quantifies their expression leveraging second-strand reads in 3' scRNA-seq data, enabling detection of alternative polyadenylation site usage.

Key Features:

• Precise polyadenylation site detection: scTail detects polyadenylation sites more precisely compared with other methods while retaining high sensitivity.
• Alternative polyadenylation site usage quantification: scTail quantifies expression of identified polyadenylation sites using second-strand reads to enable detection of alternative polyadenylation site usage.
• Integration with BRIE2: scTail can be combined with BRIE2 to discover differential alternative polyadenylation site usage in biological processes.

Scientific Applications:

• Cancer research: Combined with BRIE2, scTail discovers differential alternative polyadenylation site usage in cancers.
• Developmental biology: Combined with BRIE2, scTail discovers differential alternative polyadenylation site usage in time-series development.
• Polyadenylation site regulation studies: scTail provides critical insight into polyadenylation site regulation through analysis of alternative polyadenylation site usage.

Methodology:

scTail uses first-strand reads to identify polyadenylation sites and leverages second-strand reads to quantify expression of these sites.