scTAM-seq

scTAM-seq profiles targeted single-cell DNA methylation at single-CpG resolution without bisulfite conversion to enable high-confidence analysis of methylation dynamics across thousands of individual cells.

Key Features:

• High Throughput and Precision: Profiles up to 650 CpGs across as many as 10,000 cells per experiment, providing single-CpG resolution at large cellular scale.
• Low Dropout Rate: Achieves an approximately 7% dropout rate, supporting reliable single-cell methylation calls.
• Bisulfite-Free Approach: Performs DNA methylation profiling without bisulfite conversion, avoiding biases introduced by DNA conversion.
• Resolution of Complex Biological Processes: Resolves DNA methylation dynamics across B-cell differentiation in blood and bone marrow and identifies intermediate differentiation states.
• Integration with Cell Atlas Data: Combines DNA methylation profiling with surface-protein expression data to facilitate integration with cell atlas datasets.

Scientific Applications:

• Epigenetic Research: Enables high-resolution studies of DNA methylation regulation in development, differentiation, and disease at single-CpG and single-cell levels.
• Immunology Studies: Supports detailed analysis of B-cell differentiation and epigenetic states in immunological research.
• Cellular Heterogeneity Analysis: Dissects cellular diversity in complex tissues by detecting subtle single-cell methylation differences.

Methodology:

Targeted bisulfite-free single-cell DNA methylation profiling measuring up to 650 CpGs per experiment combined with surface-protein expression analysis; reported dropout rate ~7%.