scTEA-db

scTEA-db catalogs novel terminal exon isoforms from 53,069 publicly available human single-cell transcriptomes to characterize mRNA isoform diversity and alternative 3' UTR and protein-coding sequence variation that affect mRNA stability, translation, and localization.

Key Features:

• Novel Terminal Exon Identification: Identification of 12,063 previously unannotated terminal exons from the analysis of 53,069 publicly available single-cell transcriptomes.
• Tissue-Specific Inclusion: Observed inclusion of novel terminal exons is more tissue-specific compared to already annotated terminal exons.
• Gene Association: Novel terminal exons are linked to 5,538 human genes, including 110 known cancer driver genes.
• Intronic 3' End Discovery: Analysis revealed that approximately 25% of experimentally observed RNA 3' ends map to regions previously classified as intronic.

Scientific Applications:

• Cell Identity and Function Studies: Provides access to novel terminal exon isoforms for investigating how alternative 3' UTRs and coding-sequence variation contribute to cell identity and function.
• Cancer Research: Enables exploration of novel exon usage in 110 known cancer driver genes to aid in identification of potential biomarkers or therapeutic targets.

Methodology:

Analysis of 53,069 publicly available human single-cell transcriptomes was performed to identify terminal exon isoforms and 3' end positions, yielding 12,063 novel terminal exons and showing that ~25% of observed RNA 3' ends map to previously annotated intronic regions.