SensiPath

SensiPath identifies multi-step enzymatic metabolic pathways that convert target compounds into known effectors of allosteric transcription factors to enable detection by genetically encoded biosensors in prokaryotic systems.

Key Features:

• Enlarging Detectable Compounds: Screens for multi-step enzymatic transformations that convert non-detectable compounds into detectable effectors of transcription factors.
• Signature Descriptor Encoding: Encodes reactions using signature descriptors to represent biochemical transformations and enable pathway exploration.
• Mapping to Transcription-Factor Effectors: Identifies putative biochemical pathways that link target compounds to known effectors of allosteric transcription factors to leverage existing genetically encoded biosensors.

Scientific Applications:

• Whole-Cell Biosensors: Expands the range of analytes detectable by whole-cell biosensors in prokaryotes by converting analytes into transcription-factor effectors.
• Metabolic Engineering: Facilitates selection and regulation of high-producer strains by enabling detection of intracellular metabolic intermediates.

Methodology:

Reactions are encoded through signature descriptors and screened for multi-step enzymatic transformations that convert target compounds into known transcription-factor effectors.