SeptiSearch

SeptiSearch aggregates gene sets and associated metadata from transcriptomics studies to support systems-level analyses of sepsis and the identification of diagnostic gene signatures.

Key Features:

• Compendium of Gene Sets: A curated collection of 103 unique gene sets from 74 transcriptomics studies related to acute infection, sepsis, and severe sepsis, encompassing 20,899 unique genes identified via differential expression analyses.
• Extensive Metadata Integration: Each gene set is annotated with study-level metadata including patient group comparisons, sample collection time points, tissue types, and other study parameters.
• Identification of Molecular Mechanisms: Frequently described genes are highlighted alongside associated molecular mechanisms such as neutrophil degranulation, second messenger molecule generation, IL-4 and -13 signaling, and IL-10 signaling.

Scientific Applications:

• Gene Expression Analysis: Compare and validate in-house gene expression results against the curated gene sets to support marker or target identification.
• Pathway Exploration: Investigate molecular pathways commonly associated with sepsis to inform mechanistic hypotheses.
• Cross-Study Comparisons: Perform aggregated cross-study comparisons to enhance robustness and reproducibility of sepsis-related findings.

Methodology:

Compilation of gene sets from high-throughput transcriptomics studies, identification of genes via differential expression analyses, annotation of gene sets with study metadata, and aggregation/highlighting of frequently reported genes and associated pathways (neutrophil degranulation, second messenger molecule generation, IL-4 and -13 signaling, IL-10 signaling).