SeqAcademy

SeqAcademy provides a pipeline for processing and analyzing RNA-Seq and ChIP-Seq datasets to demonstrate computational workflows from raw sequencing reads to biological interpretation.


Key Features:

  • Supported Data Types: Analysis of RNA sequencing (RNA-Seq) and chromatin immunoprecipitation sequencing (ChIP-Seq) datasets.
  • Public Data Utilization: Use of publicly available RNA-Seq and ChIP-Seq datasets for practical analyses.
  • Integration of Bioinformatics Tools: Combination of popular bioinformatics tools into preconfigured computational workflows for read-to-interpretation analyses.
  • Biological Use Case: Inclusion of a biological example based on a previously published yeast experiment to demonstrate application of analytical steps.
  • Analysis Steps Covered: Coverage of core processing steps including quality control, alignment, quantification, and visualization of sequencing data.

Scientific Applications:

  • Educational Training: Demonstrating standard RNA-Seq and ChIP-Seq computational workflows for training and methodological familiarization.
  • Method Demonstration: Illustrating application of quality control, alignment, quantification, and visualization methods on real datasets, including a yeast use case.
  • Data Interpretation Foundation: Providing a foundation for interpreting public RNA-Seq and ChIP-Seq data and for developing downstream biological hypotheses.

Methodology:

Workflow steps explicitly include quality control, alignment, quantification, and visualization of RNA-Seq and ChIP-Seq sequencing data.

Topics

Details

License:
MIT
Tool Type:
command-line tool, library
Operating Systems:
Linux, Windows, Mac
Programming Languages:
R, Python
Added:
8/11/2018
Last Updated:
12/10/2018

Operations

Publications

Ather SH, Awe OI, Butler TJ, Denka T, Semick SA, Tang W, Busby B. SeqAcademy: an educational pipeline for RNA-Seq and ChIP-Seq analysis. F1000Research. 2018;7:628. doi:10.12688/f1000research.14880.1. PMID:33014338. PMCID:PMC7525341.

Documentation

Links