SeqAcademy
SeqAcademy provides a pipeline for processing and analyzing RNA-Seq and ChIP-Seq datasets to demonstrate computational workflows from raw sequencing reads to biological interpretation.
Key Features:
- Supported Data Types: Analysis of RNA sequencing (RNA-Seq) and chromatin immunoprecipitation sequencing (ChIP-Seq) datasets.
- Public Data Utilization: Use of publicly available RNA-Seq and ChIP-Seq datasets for practical analyses.
- Integration of Bioinformatics Tools: Combination of popular bioinformatics tools into preconfigured computational workflows for read-to-interpretation analyses.
- Biological Use Case: Inclusion of a biological example based on a previously published yeast experiment to demonstrate application of analytical steps.
- Analysis Steps Covered: Coverage of core processing steps including quality control, alignment, quantification, and visualization of sequencing data.
Scientific Applications:
- Educational Training: Demonstrating standard RNA-Seq and ChIP-Seq computational workflows for training and methodological familiarization.
- Method Demonstration: Illustrating application of quality control, alignment, quantification, and visualization methods on real datasets, including a yeast use case.
- Data Interpretation Foundation: Providing a foundation for interpreting public RNA-Seq and ChIP-Seq data and for developing downstream biological hypotheses.
Methodology:
Workflow steps explicitly include quality control, alignment, quantification, and visualization of RNA-Seq and ChIP-Seq sequencing data.
Topics
Details
- License:
- MIT
- Tool Type:
- command-line tool, library
- Operating Systems:
- Linux, Windows, Mac
- Programming Languages:
- R, Python
- Added:
- 8/11/2018
- Last Updated:
- 12/10/2018
Operations
Publications
Ather SH, Awe OI, Butler TJ, Denka T, Semick SA, Tang W, Busby B. SeqAcademy: an educational pipeline for RNA-Seq and ChIP-Seq analysis. F1000Research. 2018;7:628. doi:10.12688/f1000research.14880.1. PMID:33014338. PMCID:PMC7525341.
Documentation
Training material
http://www.seqacademy.org/p/tutorial.html