SeqBuster

SeqBuster performs profiling and characterization of small RNA sequencing datasets from high-throughput sequencing to identify and describe microRNA (miRNA) variants (isomiRs) and annotate small RNA content.

Key Features:

• Small RNA processing: Processes large-scale small RNA datasets generated by high-throughput sequencing technologies.
• Pre-analysis mapping options: Provides flexible pre-analysis read-mapping options to configure mapping strategies before downstream analysis.
• Modular analysis: Implements multiple modules for data manipulation and analysis of small RNA content.
• IsomiR characterization: Performs in-depth and exhaustive characterization of microRNA (miRNA) variants (isomiRs).
• Custom-database annotation: Annotates small RNA reads and identified variants against custom databases.

Scientific Applications:

• IsomiR profiling: Discovery and detailed description of miRNA variants (isomiRs) in small RNA-seq datasets.
• Stem cell differentiation studies: Analysis of miRNA variants associated with human embryonic stem cell differentiation.
• Biological mechanism and disease research: Identification of miRNA-variants implicated in physiological and pathological processes.
• Database-driven annotation: Functional and comparative analyses of small RNAs using custom database annotation.

Methodology:

Pre-analysis read mapping, modular data manipulation and analysis, isomiR identification and exhaustive characterization, and annotation of reads/variants against custom databases.