SeqLengthPlot

SeqLengthPlot visualizes and retrieves sequence lengths from FASTA files, generating statistical summaries and dynamic visualizations for nucleotide and protein sequence length profiling in genomics and proteomics.

Key Features:

• Statistical Summaries: Generates comprehensive statistical summaries of sequence lengths from input FASTA files.
• Sequence Filtering: Filters sequences based on a user-defined length threshold.
• Sequence Retrieval: Automatically retrieves and splits sequences into two distinct output files based on a tunable, user-defined sequence length splitting point.
• Dynamic Visualizations: Produces plots and dynamic visualizations of sequence length distributions.
• Dual Sequence Type Support: Processes both nucleotide and protein FASTA files.

Scientific Applications:

• Genomics: Profiles nucleotide sequence lengths for quality assessment and assembly evaluation.
• Proteomics: Analyzes protein sequence length distributions for comparative and functional studies.
• Sequence Quality Control: Identifies and separates sequences above or below critical length thresholds for downstream analysis.
• Assembly Benchmarking: Complements N50, median, and average length metrics with total sequence count and graphical representations.

Methodology:

SeqLengthPlot is implemented in Python. It reads FASTA files, computes sequence length statistics, filters and splits sequences at a user-defined length threshold into two output files, and generates dynamic visualizations of the length distributions.