SeqLengthPlot v2.0

SeqLengthPlot v2.0 visualizes and retrieves sequence lengths from FASTA files, splitting sequences by length threshold and generating distribution plots with statistical summaries for genomics and proteomics analyses.

Key Features:

• Sequence splitting by length threshold: Splits input FASTA files (nucleotide and protein) into two distinct files based on a customizable sequence length cutoff, retrieving both shorter and longer sequences simultaneously.
• Length distribution plotting: Automatically generates graphical representations of sequence length distributions.
• Comprehensive statistical summaries: Provides detailed statistics including number of sequences, average, minimum, and maximum lengths, N50, and median lengths.

Scientific Applications:

• Genomics sequence profiling: Enables accurate sequence length profiling for genomic analyses requiring assessment of contig or read length distributions.
• Proteomics sequence profiling: Supports length-based analysis of protein sequences from FASTA files.
• Assembly quality assessment: Facilitates evaluation of sequence assemblies through N50, median, average, and distribution metrics.

Methodology:

SeqLengthPlot v2.0 is implemented in Python and processes FASTA files through a command-line interface.