SeqSolve

SeqSolve analyzes Next Generation Sequencing (NGS) data to profile small RNAs (sRNAs) and discover novel RNA species and biochemical pathways.

Key Features:

• Comprehensive sRNA Profiling: Profiles and characterizes sRNAs, including detection of non-genomically encoded 5' poly(U) tails.
• Novel Transcript Discovery: Detects novel transcripts and predicts previously unrecognized biochemical processes, including evidence for an unknown capping process on cleaved RNA.
• Pathway Prediction and Characterization: Predicts and characterizes novel endogenous biochemical pathways, including indications of a pathway capable of copying RNA enriched in genes encoding translational machinery.
• Gene Flanking Analysis: Identifies sense, 3' polyadenylated sRNAs flanking genes that are likely capped.

Scientific Applications:

• Functional RNA Identification: Distinguishes functional sRNAs from degradation products to support studies of RNA regulatory roles.
• Pathway Elucidation: Enables prediction and characterization of novel biochemical pathways revealed by sRNA profiles.
• Translational Machinery Insights: Highlights enrichment of sRNAs associated with transcripts encoding components of the translational machinery.

Methodology:

Applies advanced bioinformatics algorithms to process and analyze NGS data with a focus on sRNA species and comprehensive profiling to detect features such as 5' poly(U) tails and 3' polyadenylation; integrates genetic and in vitro study findings for validation.