SignaLink3

SignaLink3 provides manually curated, tissue- and compartment-specific signaling network data for Homo sapiens, Caenorhabditis elegans, Danio rerio, and Drosophila melanogaster to support pathway, regulatory and disease-association analyses.

Key Features:

• Extensive Interaction Data: Contains over 700,000 protein-protein interactions for Homo sapiens, including more than 400,000 newly added human interactions.
• Model Organism Coverage: Provides regulatory information for Caenorhabditis elegans and Danio rerio and is described as the most comprehensive resource for Drosophila melanogaster.
• Integration of Gene Expression and Localization Data: Integrates gene expression data with subcellular localization to enable tissue- or compartment-specific pathway interaction analysis.
• Regulatory and Disease Annotations: Includes interactions, regulatory relationships, subcellular localization and disease-association annotations for signaling components.
• Manual Curation and Multi-database Integration: Assembles data through manual curation from multiple source databases.
• Data Formats: Provides data in CSV, PSI-MI TAB, and SQL formats.

Scientific Applications:

• Pathway Analysis: Enables detailed exploration of signaling pathways and their regulatory interactions across tissues and compartments.
• Disease Association Studies: Supports investigation of how signaling network disruptions relate to disease-associated genes and pathways.
• Model Organism Research: Facilitates comparative and translational studies using comprehensive signaling and regulatory data for key model organisms.

Methodology:

Manually curated data were integrated from multiple databases to compile protein-protein interactions, regulatory relationships, subcellular localization, gene expression and disease-association annotations.