SimpatiQCo

SimpatiQCo monitors LC-MS system performance to provide longitudinal quality control for proteomic and quantitative studies on Orbitrap-family instruments.

Key Features:

• Instrument Compatibility: SimpatiQCo supports LTQ Orbitrap, Q-Exactive, LTQ FT, and LTQ instruments.
• Centralized Data Processing: Processes QC data from multiple instruments on a centralized server.
• QC Metrics Calculation: Computes LC, ESI, and MS metrics including peptide elution times, peak widths, intensities, total ion current (TIC), and sensitivity to evaluate overall LC-MS performance.
• Robust Statistical Analysis: Applies robust statistics to learn acceptable performance ranges from uploaded QC data.
• Longitudinal Monitoring: Enables per-run monitoring and longitudinal plotting of QC metrics to detect trends and anomalies over time.
• Database Storage: Stores computed metrics and results in a database for historical analysis.

Scientific Applications:

• Proteomics and Quantitative Studies: Ensures consistent LC-MS performance for high-throughput proteomic analyses and quantitative experiments.
• Instrument Performance Surveillance: Supports longitudinal monitoring to identify instrument degradation or acquisition issues before they impact data quality.

Methodology:

Centralized server-side processing computes LC, ESI, and MS QC metrics (peptide elution times, peak widths, intensities, TIC, sensitivity), applies robust statistical learning to define acceptable performance ranges, stores metrics in a database, and enables longitudinal plotting.