SLqPCR

SLqPCR analyzes quantitative PCR (qPCR) and high-throughput expression data to provide reproducible workflows for data import, normalization, quality assessment, and downstream interpretation within the Bioconductor R environment.


Key Features:

  • Bioconductor R package: Implemented as an R package within the Bioconductor ecosystem to leverage interoperable statistical and bioinformatic infrastructure.
  • qPCR and high-throughput support: Handles quantitative PCR (qPCR) measurements and high-throughput expression datasets.
  • Reproducible workflows: Provides reproducible pipelines for data import, normalization, quality assessment, and downstream interpretation.
  • Interoperability: Integrates with other Bioconductor packages to combine qPCR results with broader genomic datasets.
  • Software quality practices: Uses community-driven development practices including formal code review and continuous automated testing to ensure reliability and compatibility.

Scientific Applications:

  • Gene-expression studies: Analysis of qPCR-derived measurements for gene-expression experiments.
  • Integrative genomic analyses: Combining qPCR results with other genomic datasets through Bioconductor interoperability for broader interpretation.

Methodology:

Computational steps explicitly include data import, normalization, quality assessment, and downstream interpretation implemented in an R/Bioconductor package.

Topics

Collections

Details

License:
GPL-2.0
Tool Type:
command-line tool, library
Operating Systems:
Linux, Windows, Mac
Programming Languages:
R
Added:
1/17/2017
Last Updated:
12/10/2018

Operations

Publications

Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M. Orchestrating high-throughput genomic analysis with Bioconductor. Nature Methods. 2015;12(2):115-121. doi:10.1038/nmeth.3252. PMID:25633503. PMCID:PMC4509590.

Documentation

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