smDynamicsAnalyzer

smDynamicsAnalyzer analyzes single-molecule tracking (SMT) datasets from single-molecule imaging (SMI), particularly TIRFM acquisitions in living cells, to extract and quantify dynamic parameters of membrane proteins.

Key Features:

• Macro implementation: Implemented as an open-source macro for processing SMT/SMI datasets.
• Comprehensive dynamics extraction: Determines diffusion dynamics, oligomer size distribution, and particle density changes from SMI data.
• Interaction estimation: Performs dual-color SMI analysis to estimate affinities and on/off-rates of protein–protein interactions.

Scientific Applications:

• GPCR dynamics: Measures drug-activated dynamics of G protein-coupled receptors (GPCRs), such as the metabotropic glutamate receptor 3 (mGluR3).
• Membrane protein research: Provides quantitative insights into diffusion and interaction dynamics relevant to cellular signaling pathways and membrane biology.

Methodology:

The smDynamicsAnalyzer processes SMI datasets through a structured workflow including data organization, parameter extraction, and quantitative analysis.