SMETHILLIUM

SMETHILLIUM normalizes spatial background noise in Illumina InfinIUM HumanMethylation BeadChip arrays to improve the accuracy of DNA methylation measurements.

Key Features:

• Spatial Normalization: Employs a non-parametric normalization method specifically developed to correct spatial background noise inherent in Illumina HumanMethylation27 BeadChip data, improving signal-to-noise ratio and accuracy of methylation status predictions.
• Performance Superiority: Demonstrated superior performance to BeadStudio in predicting methylation state using three fully methylated and three fully unmethylated DNA samples.

Scientific Applications:

• Epigenetic Research: Provides more accurate methylation data for studies of gene regulation, disease mechanisms (e.g., cancer), and developmental biology.
• Genomic Studies: Enables quantification of methylation state across 27,578 loci spanning 14,495 genes.

Methodology:

Non-parametric normalization of spatial background noise that does not rely on predefined statistical distributions.