SNAT

SNAT provides a comprehensive transcriptomic atlas of the mouse sciatic nerve by integrating bulk RNA sequencing and single-cell RNA sequencing to enable cell-type-resolved transcriptional profiling across developmental stages.

Key Features:

• Multi-layered transcriptome analysis: Integration of bulk RNA-seq and single-cell RNA-seq datasets covering perinatal to adult stages.
• Bulk RNA sequencing across development: Bulk RNA-seq captures overall gene expression profiles of developing mouse sciatic nerves up to adulthood.
• Schwann cell-focused studies: Focused transcriptomic analyses characterize Schwann cell transcriptional dynamics across postnatal stages.
• Single-cell RNA sequencing (targeted and comprehensive): scRNA-seq encompasses the full spectrum of sciatic nerve cell types in perinatal and adult phases.
• Cell-type coverage: Includes axon-associated Schwann cells, endothelial cells, immune-associated cells, perineurium barrier-forming cells, and connective tissue-resident cells in intra-fascicular endoneurium and inter-fascicular epineurium.
• Integrated reference transcriptome: Diverse datasets are combined to produce a comprehensive reference of cellular composition and gene expression in the sciatic nerve.

Scientific Applications:

• Reference atlas for gene expression: Provides a reference for gene expression patterns across sciatic nerve cell types.
• Developmental transcriptional studies: Enables analysis of transcriptional dynamics during postnatal development, particularly in Schwann cells.
• Cellular composition characterization: Supports identification and comparison of cell-type-specific expression in endoneurium and epineurium compartments.
• Research into nerve biology and disease: Facilitates studies of peripheral nerve biology, function, and investigations relevant to peripheral nerve disorders.

Methodology:

Bulk RNA sequencing of developing mouse sciatic nerves and single-cell RNA sequencing (targeted and comprehensive) across perinatal and adult stages, including focused Schwann cell studies, with integration of these datasets into the atlas.