Solubis 2

Solubis 2 optimizes protein solubility by identifying aggregation-prone linear segments and proposing minimal point mutations that reduce aggregation while preserving thermodynamic stability.

Key Features:

• Detection of aggregation-prone segments: Solubis 2 identifies linear segments within protein sequences that are prone to aggregation.
• Identification of mutations: It proposes minimal point mutations that eliminate aggregation propensity of identified segments while preserving thermodynamic stability.
• Applicability to globular proteins and antibodies: Applicable to a wide range of globular proteins, including antibodies, when structural information is available for precise targeting.

Scientific Applications:

• Increased in vitro protein solubility: By reducing aggregation-prone segments, Solubis 2 increases protein solubility in vitro to facilitate purification and characterization.
• Improved recombinant protein production yields: Maintaining stability while enhancing solubility leads to improved yields during recombinant protein production.

Methodology:

Computational prediction of aggregation-prone regions from protein sequences, proposal of minimal point mutations to mitigate aggregation without altering overall thermodynamic stability, and use of available structural data to guide mutation targeting.