SpeedyGenesXL

SpeedyGenesXL generates ultralarge bespoke variant gene libraries to enable systematic exploration of protein sequence space for directed evolution and protein engineering.

Key Features:

• Automation and high-throughput capability: Automates production of diverse gene libraries to increase throughput in library preparation.
• Supported library types: Generates wild-type genes, Boolean OR, combinatorial, and combinatorial-OR-type libraries.
• Bespoke ultralarge variant synthesis: Enables creation of application-specific ultralarge variant gene libraries.
• Cost reduction in library sourcing: Reduces reliance on commercial libraries for large-scale directed evolution experiments.

Scientific Applications:

• Protein engineering: Facilitates directed evolution to improve catalytic efficiency (k_cat), thermostability, and organic solvent tolerance.
• Variant discovery and screening: Enables systematic exploration of protein sequence space using ultralarge libraries for identification of desirable variants.

Methodology:

Builds upon the SpeedyGenes methodology for generating diverse genetic libraries and automates simultaneous production of multiple bespoke libraries.