SpliceAid

SpliceAid provides a curated database of experimentally validated RNA target motifs and sequences bound by human splicing proteins to support analysis of protein–RNA interactions in RNA splicing.

Key Features:

• Extensive database of experimental data: Compiles experimentally validated RNA target sequences and motifs recognized by human splicing proteins derived from literature curation.
• Score matrix annotation refinement: Supplies experimentally derived target sequences to address overestimation errors of score matrices used to annotate RNA-binding protein targets.

Scientific Applications:

• Splicing mechanism analysis: Enables analysis of sequence-specific interactions between splicing proteins and RNA to study splicing regulation.
• Gene expression and disease investigation: Supports investigation of regulatory roles of splicing proteins in gene expression and their implications in diseases.
• Experimental design and validation: Provides reference sequences to design experiments and validate hypotheses about protein–RNA interactions.
• Therapeutic target exploration: Facilitates exploration of potential therapeutic targets related to splicing anomalies.

Methodology:

Data were collected via a comprehensive literature search to identify experimentally assessed RNA sequences bound by human splicing proteins, then curated and organized into a structured database format.