SpliceAid-F

SpliceAid-F catalogs experimentally validated interactions between human RNA-binding splicing regulatory proteins and RNA sites to support studies of splicing regulation.

Key Features:

• Comprehensive Data Collection: Aggregates experimentally validated data on 71 human RNA-binding splicing regulatory proteins, including functional domains, protein and chemical interactors, and expression data.
• RNA–Splicing Factor Interactions: Documents 4,227 validated RNA–splicing factor interactions with details on RNA-binding sites, genomic coordinates, associated genes, and effects on splicing.
• RNA Binding Sites: Catalogs 2,590 RNA-binding sites for analysis of sequence-level splicing regulation.
• No-Binding Data: Contains 1,141 documented 'no-binding' sites where no RNA–splicing factor binding was observed under specific experimental conditions.
• Experimental Contexts: Records experimental procedures used to validate interactions and provides bibliographic references for each interaction.
• Contextual Information: Includes data on cellular contexts and conditions under which binding was tested.

Scientific Applications:

• Understanding Splicing Mechanisms: Enables analysis of protein–RNA networks and regulatory determinants involved in pre-mRNA splicing.
• Explaining Observed Splicing Patterns: Supports interpretation of tissue- or condition-specific splicing variation by linking interactions and contextual data.
• Mutation Impact Analysis: Facilitates assessment of how mutations in regulatory elements may alter splicing by referencing validated binding and no-binding sites.

Methodology:

Data were obtained through hand-curated screenings of published literature and existing databases.