splitcode

splitcode enables flexible and efficient parsing, interpreting, and editing of sequencing reads to facilitate preprocessing of libraries constructed for single-cell and bulk sequencing assays.

Key Features:

• Flexible Parsing: Processes synthetic constructs including sequencing adapters, barcodes, and unique molecular identifiers from next-generation sequencing libraries.
• Efficient Editing: Performs reproducible preprocessing of reads across a large array of single-cell and bulk sequencing assays.
• Technical Sequence Interpretation: Extracts and analyzes experimental information contained within technical sequences essential for interpreting sequencing assay results.

Scientific Applications:

• Single-Cell Sequencing Preprocessing: Facilitates preprocessing of reads from single-cell sequencing libraries containing barcodes and unique molecular identifiers.
• Bulk Sequencing Preprocessing: Supports preprocessing of reads from bulk sequencing assays with diverse synthetic constructs.
• Barcode and UMI Processing: Enables extraction and analysis of barcodes and unique molecular identifiers critical for experimental interpretation.

Methodology:

splitcode parses, interprets, and edits technical sequences including sequencing adapters, barcodes, and unique molecular identifiers from next-generation sequencing reads.