SPRD

SPRD compiles technical details from 5,140 publications of surface plasmon resonance (SPR) experiments to enable analysis of experimental conditions for biomolecular interaction studies such as protein-protein, protein-DNA, and protein–small molecule binding and applications in small molecule drug development and antibody characterization.

Key Features:

• Publication corpus: Contains technical details extracted from 5,140 publications reporting SPR experiments.
• Experimental variable catalog: Catalogs variables that influence SPR data including buffer composition, sensor chip type, coupling chemistry for immobilization, and surface regeneration conditions.
• Immobilization and detection details: Documents immobilization approaches (microchip immobilization, amine coupling, and capture methods) and detection context (microfluidic delivery and real-time measurement of changes in total mass on the chip).
• Common-practice analysis: Analyzes preferred experimental conditions across laboratories and highlights common practices such as amine coupling and use of CM5 chips while documenting alternative chips and capture methods.
• Application-focused summaries: Summarizes conditions used in studies of protein-protein, protein-DNA, and protein–small molecule binding relevant to screening, validation, and characterization workflows.

Scientific Applications:

• Experimental planning and optimization: Inform design and optimization of SPR assays by providing empirical distributions of buffer, chip, coupling, and regeneration conditions.
• Small molecule drug development: Support selection of SPR conditions for screening and validation of small molecule–protein interactions in drug discovery.
• Antibody characterization: Guide selection of immobilization and regeneration strategies for antibody binding studies by SPR.
• Method development and troubleshooting: Aid troubleshooting and optimization of immobilization chemistries, capture methods, and surface regeneration protocols.

Methodology:

Extraction of technical details from 5,140 publications and analysis of reported experimental conditions across studies.