SPRINT

SPRINT identifies RNA editing sites (RESs) directly from RNA-seq data without requiring single nucleotide variant (SNV) filtering using SNP databases and detects hyper RESs by analyzing remapped reads.

Key Features:

• SNP-Free Detection: Identifies RESs directly from RNA-seq data without filtering SNVs using SNP databases.
• Hyper RES Detection: Detects hyper RESs by analyzing remapped reads.
• Automated Workflow: Provides an automated workflow applicable to any RNA-seq dataset with an available reference genome sequence.
• Experimental Validation: Validated using RNA-seq data from samples in which genes encoding RNA editing enzymes are knocked down or over-expressed.

Scientific Applications:

• Cross-tissue and Cross-species Analysis: Used to explore RNA editing across different tissues and species.
• Developmental Studies: Applied to study RNA editing during development of the mouse embryonic central nervous system.

Methodology:

Detects RESs from RNA-seq without relying on SNP database filtering of SNVs and identifies hyper RESs by analyzing remapped reads.