SR-Tesseler

SR-Tesseler segments and quantifies molecular organization from localization-based super-resolution microscopy by constructing Voronoi tessellations from localized molecule coordinates.

Key Features:

• Voronoi Tessellation-Based Segmentation: Constructs polygonal Voronoi tessellations from localization coordinates to segment molecular assemblies.
• Multi-Scale Quantification: Quantifies protein arrangements across scales from whole-cell structures down to small clusters composed of a few fluorescent markers.
• Robustness Against Variability: Provides measures that are insensitive to variations in cell shape, molecular organization, background noise, and imaging artifacts to enable unbiased comparisons.
• Automatic Analysis: Performs automated segmentation and quantification based on the tessellation framework.

Scientific Applications:

• Protein organization analysis: Quantitative analysis of complex protein organizations using localization-based super-resolution microscopy data.
• Validation and benchmarking: Application to both simulated and experimental localization datasets for method validation.
• Fluorophore-labeled protein studies: Analysis of proteins labeled with genetically encoded fluorescent proteins or organic fluorophores.

Methodology:

Segmentation is performed by constructing Voronoi tessellations from localized molecular coordinates, and quantification is derived from the resulting tessellation-based segments.