SRA

SRA archives raw sequencing reads and alignment information from next-generation sequencing platforms to preserve experimental data and enable reproducibility.

Key Features:

• Raw data and alignments: Stores raw sequencing reads and alignment information from submitted experiments.
• INSDC collaboration: Operates as part of the International Nucleotide Sequence Database Collaboration (INSDC) with NCBI, EBI, and DDBJ.
• Supported sequencing technologies: Accepts data from Roche 454 GS System®, Illumina Genome Analyzer®, Applied Biosystems SOLiD System®, Helicos Heliscope®, Complete Genomics®, and Pacific Biosciences SMRT® platforms.
• Metadata structures: Maintains structured metadata describing content and experimental context.
• Submission file formats: Defines and updates standardized submission file formats and recommended data submission levels.
• Data growth adaptation: Evolves metadata and submission formats to accommodate increasing sequencing data volumes.
• Content and structure information: Provides detailed descriptions of archive content, data organization, and supported platform information.

Scientific Applications:

• Data archiving: Serves as a primary repository for long-term storage of next-generation sequencing datasets.
• Reproducibility and reuse: Preserves raw reads and alignments to enable independent reanalysis and reproducible research.
• Compliance: Supports fulfillment of journal and funding agency data deposition requirements.
• Cross-platform analyses: Enables comparative studies across data generated by Roche 454, Illumina, SOLiD, Helicos, Complete Genomics, and PacBio technologies.

Methodology:

Storage of raw sequencing reads and alignment information, and maintenance/updating of metadata structures and standardized submission file formats.