SRCP

SRCP annotates and quantifies circular RNAs (circRNAs) to enable sensitive detection, annotation, and comparative analysis of differentially expressed and functionally relevant circRNAs across multiple species.

Key Features:

• Two-Step Process: Employs a dual-phase workflow that first annotates validated circRNAs and then quantifies their expression.
• Enhanced Sensitivity and Comprehensiveness: Engineered to detect a broader spectrum of differentially expressed circRNAs with increased sensitivity compared to individual pipelines.
• Cross-Species Validation: Includes a comprehensive collection of validated circRNAs from five organisms, including humans, to support comparative studies.
• Integrative Annotation and Quantification: Uses an integrative approach combining validated annotation datasets with precise quantification steps.

Scientific Applications:

• CircRNA Annotation and Quantification: Provides annotation and expression quantification of circRNAs, which are non-coding RNAs involved in cellular regulatory processes.
• Identification of Functional CircRNAs: Applied to identify subsets of circRNAs that interact with the miRNA-effector protein AGO2, including analyses in human brain samples.
• Differential Expression Studies: Enables exploration of circRNA differential expression across physiological or pathological conditions.

Methodology:

SRCP implements a two-step integrative computational workflow that combines annotation of validated circRNAs with subsequent precise quantification of their expression.