Start2Fold

Start2Fold provides a curated database of hydrogen/deuterium exchange (HDX) data for analyzing protein folding pathways and stability at residue-level resolution.

Key Features:

• Curated HDX datasets: Aggregates HDX data extracted from peer-reviewed literature to assemble quality-controlled experimental datasets.
• Hierarchical framework: Organizes entries within a hierarchical framework to enable systematic comparison and reinterpretation of datasets.
• Residue classification: Annotates residues with exchange protection levels and records associated experimental procedures.
• Residue-to-structure mapping: Maps annotated residue groups onto corresponding three-dimensional protein structures.

Scientific Applications:

• Interpreting protein behavior: Provides residue-level HDX data to elucidate folding mechanisms, stability determinants, and conformational dynamics.
• Developing computational methods: Supports development and benchmarking of computational predictors for folding, stability, and solvent protection.
• Enhancing experimental techniques: Enables comparison and refinement of solvent exchange–based experimental protocols and data interpretation.

Methodology:

Data extraction from peer-reviewed literature, manual curation, hierarchical organization of entries, and classification of residues by protection levels observed in HDX experiments.