StatSigMA

StatSigMA evaluates the statistical significance of multiple sequence alignments (MSAs) of nucleotide and amino acid sequences to detect non-homologous contamination.

Key Features:

• Statistical Assessment: Provides quantitative significance scores for MSAs to assess alignment reliability, analogous to BLAST E-values for pairwise alignments.
• Contamination Detection: Identifies and flags MSAs that include one or more non-homologous (unrelated) sequences.
• Comparative Analysis: Enables statistical comparison of outputs from different multiple alignment tools.

Scientific Applications:

• Homology Determination: Distinguishes homologous from non-homologous sequences to support phylogenetic and evolutionary analyses.
• Tool Evaluation: Allows researchers to evaluate and compare the performance of multiple alignment tools using statistical significance metrics.

Methodology:

Analyzes MSAs to detect statistical anomalies and computes significance scores for alignments.