STRhub

STRhub catalogs forensic short tandem repeat markers, population allele frequencies, and summary statistics from capillary electrophoresis and sequencing datasets to support education, teaching, and research in forensic STR genetics.

Key Features:

• STR Marker Catalog: Provides 228 STR markers including CODIS core, other autosomal, X-STRs, and Y-STRs with GRCh38/GRCh37 coordinates, repeat motifs, ISFG/STRNaming sequence structures, and MPS kit coverage from STRidER's FSSG.
• Population Allele Frequency Data: Contains population allele frequencies and summary statistics from capillary electrophoresis (pop.STR / SP-SMART) and sequencing (1000 Genomes Project) datasets, explorable in charts and downloadable as tables.
• DNA Mixture Simulator: Simulates DNA mixtures and contrasts capillary electrophoresis and next-generation sequencing behaviour.
• STR Motif Explorer: Explores STR motifs over the ISFG minimum range.
• FASTA Generator: Generates FASTA sequences with configurable flanking regions.
• Embedded Genome Viewer: Includes an embedded IGV.js viewer with 1000 Genomes demo reads.

Scientific Applications:

• Forensic STR Genetics Education: Supports education, teaching, and research in forensic short tandem repeat genetics.
• Population Genetics Analysis: Enables exploration of population allele frequencies and summary statistics from capillary electrophoresis and sequencing datasets.
• NGS and CE Comparison: Facilitates comparison of capillary electrophoresis and next-generation sequencing behaviour through DNA mixture simulation.

Methodology:

STRhub integrates data from STRidER's FSSG for MPS kit coverage, pop.STR / SP-SMART for capillary electrophoresis population data, and the 1000 Genomes Project for sequencing-based allele frequencies.